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时间: 07-05 06:25
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时间: 07-05 06:22
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| Andy |
时间: 07-05 06:21
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K562 cells were engineered to express （a） a human CD19 （having the amino acid sequence set forth in SEQ ID NO:92） （b） a Macaca mulatta （rhesus macaque （rhesus）） CD19 （having the amino acid sequence set forth in SEQ ID NO:139; Accession No. F7F486） or （c） one the three different human/rhesus chimeric CD19 molecules V1 V2 and V3 which contained membrane-proximal regions having the sequences depicted in FIG. 18A. Aside from the region depicted in FIG. 18A the remaining regions of each chimeric molecule were identical in sequence to the corresponding regions of the rhesus CD19. The results are shown in FIG. 5A indicating that FMC63 IgG competed for binding to CD19 with SJ25C1 IgG1 in this study suggesting that SJ25C1 and FMC63 bound to overlapping epitopes e.g. a common epitope of CD19. The results shown in FIG. 11 demonstrated that the clones competed with FMC63 scFv for binding to CD19-expressing cells with various IC.sub.50 values. |
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