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The term "comparable to" is used herein to mean a value that is as much as 30% less than or more than the reference value to which it is being compared. In certain embodiments the present disclosure provides a method of identifying or determining a molecule having intolerable in vivo neurotoxicity comprising measuring calcium oscillations in vitro in neuronal cells after being in contact with the molecule in the neuronal cells. The present disclosure is also directed to a method of testing or determining in vivo neurotoxicity of a molecule （e.g. polynucleotide） comprising a nucleotide sequence. Such methods are helpful to reduce unnecessary killing of animals during testing of the molecule\‘s toxicity and/or enhance the possibilities that the molecules will be safe for in vivo administration. The present disclosure provides methods for testing or determining the toxicity （e.g. in vivo acute neurotoxicity） of a molecule by measuring certain characteristics of the molecule. In one aspect the disclosure s |
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